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recombinant mouse cxcl2  (Cusabio)


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    Cusabio recombinant mouse cxcl2
    Recombinant Mouse Cxcl2, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant mouse cxcl2/product/Cusabio
    Average 93 stars, based on 15 article reviews
    recombinant mouse cxcl2 - by Bioz Stars, 2026-03
    93/100 stars

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    Image Search Results


    Reagents and tools table

    Journal: EMBO Reports

    Article Title: CMTM3 regulates neutrophil activation and aggravates sepsis through TLR4 signaling

    doi: 10.1038/s44319-024-00291-7

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: Recombinant Mouse CXCL2 Protein , R&D Systems , 452-M2.

    Techniques: Recombinant, Staining, Enzyme-linked Immunosorbent Assay, Red Blood Cell Lysis, cDNA Synthesis, SYBR Green Assay, Real-time Polymerase Chain Reaction, Isolation, Membrane, Software

    CXCR1/2 antagonism does not confer a loss of neutrophil chemotactic capacities of tumors. Following a 4-d treatment period (black = control, red = AZD, and blue = SX-682), thioglycolate-induced neutrophils (>85% pure) were isolated from the peritoneum exudates and used in the modified Boyden chamber chemotaxis assay. The chemotactic capacities of the indicated neutrophils were assessed toward the gradients by (A) CXCL2 (50 ng/mL), (B and C) AZD-treated PL and LLC tumor lysates, and (D-E) SX-682-treated PL and LLC tumor lysates. Each closed circle represents an experimental replicate from three separate biological replicates with the indicated tumor lysates (1 mg/mL) prepared from at least three different mice. The mean percentages of migrated neutrophil over total seeded cells ± s.d. Are shown. (F-I) CXCL1, CXCL2, C5a, and LTB4 contents in whole tumor lysates of the indicated tumor model and treatment were measured by ELISA and expressed as pg/mg of total protein loaded. Each open circle represents a lysate of the tumor bearing lung of mouse that were treated with vehicle control, AZD, or SX-682 for 2 weeks. The graphs show the mean ± s.d., and ordinary one-way ANOVA with Tukey’s post hoc test (* p < 0.05) was used for the statistical analysis.

    Journal: Oncoimmunology

    Article Title: CXCR1/2 antagonism inhibits neutrophil function and not recruitment in cancer

    doi: 10.1080/2162402X.2024.2384674

    Figure Lengend Snippet: CXCR1/2 antagonism does not confer a loss of neutrophil chemotactic capacities of tumors. Following a 4-d treatment period (black = control, red = AZD, and blue = SX-682), thioglycolate-induced neutrophils (>85% pure) were isolated from the peritoneum exudates and used in the modified Boyden chamber chemotaxis assay. The chemotactic capacities of the indicated neutrophils were assessed toward the gradients by (A) CXCL2 (50 ng/mL), (B and C) AZD-treated PL and LLC tumor lysates, and (D-E) SX-682-treated PL and LLC tumor lysates. Each closed circle represents an experimental replicate from three separate biological replicates with the indicated tumor lysates (1 mg/mL) prepared from at least three different mice. The mean percentages of migrated neutrophil over total seeded cells ± s.d. Are shown. (F-I) CXCL1, CXCL2, C5a, and LTB4 contents in whole tumor lysates of the indicated tumor model and treatment were measured by ELISA and expressed as pg/mg of total protein loaded. Each open circle represents a lysate of the tumor bearing lung of mouse that were treated with vehicle control, AZD, or SX-682 for 2 weeks. The graphs show the mean ± s.d., and ordinary one-way ANOVA with Tukey’s post hoc test (* p < 0.05) was used for the statistical analysis.

    Article Snippet: Tumor lysates (1 mg/mL) or mouse recombinant mouse CXCL2 (50 ng/mL; R&D; 452-M2) were prepared in HBSS and used in neuroprobe cell migration chamber with 3-µm filter.

    Techniques: Control, Isolation, Modification, Chemotaxis Assay, Enzyme-linked Immunosorbent Assay